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Cytoarchitecture of kirsten sarcoma virus-transformed rat kidney fibroblasts: Butyrate-induced reorganization within the actin microfilament network
Ist Teil von
Journal of cellular physiology, 1988-10, Vol.137 (1), p.25-34
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
1988
Link zum Volltext
Quelle
Wiley Online Library All Journals
Beschreibungen/Notizen
Murine sarcoma virus‐transformed rat fibroblasts (KNRK cells) undergo marked cytoarchitectural reorganization during in vitro exposure to sodium‐n‐butyrate (NaB) resulting in restoration of (1) a more typical fibroblastoid morphology, (2) proper cell‐to‐cell orientation, and (3) substratum adherence. Augmented cell spreading, involving > 90% of the population was a function of culture density and time of exposure to NaB (2 mM final concentration). Induced cell spreading reflected a 2.5‐ to 3.0‐fold increase in both total cellular actin content and deposition of actin into the detergent‐resistent cytoskeleton. Cytoskeletal actin deposition in response to NaB was accompanied by the formation of occasionally dense, parallel alignments of F‐actin‐containing microfilaments and by a dramatic increase in the size and incidence of actin‐enriched membrane ruffles. Long‐term NaB‐treated cells exhibited parallel orientations of microfilaments similar to those found in untransformed fibroblasts. Increased cytoskeletal actin occurred within 24 hr of NaB exposure, correlating with the initial reorganization of actin‐containing microfilaments detected microscopically, and reflected concomitant 3‐fold increases in cellular alpha‐actinin and fibronectin content. In contrast, the amount of vimentin, tropomyosin, and tubulin in NaB‐treated cells was significantly decreased. NaB‐induced morphologic restructuring of sarcoma virus‐transformed fibroblast, thus, impacts on all three basic cytoskeletal systems. Selective increases, however, were evident in particular cytoskeletal proteins (actin, alpha‐actinin, fibronectin) implicated in microfilament networking and cell spreading.