Details

Autor(en) / Beteiligte

• Seidler, Lothar
• Kaszkin, Marietta
• Kinzel, Volker

Titel

Primary Alcohols and Phosphatidylcholine Metabolism in Rat Brain Synaptosomal Membranes via Phospholipase D

Ist Teil von

• Pharmacology & toxicology, 1996-04, Vol.78 (4), p.249-253

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1996

Quelle

MEDLINE

Beschreibungen/Notizen

• : Phospholipase D of rat brain synaptosomal membranes was tested with phosphatidylcholine as the substrate for its specificity in the use of primary alcohols as transphosphatidylation co‐substrates. The efficiency of the reaction was related to the hydrophobicity and the membrane penetrating capacity of the alcohol molecule. Phosphatidylalcohol formation could be detected up to 1‐octanol but not for alcohols with longer hydrocarbon chains (C9, C10). With increasing alcohol concentration the transphosphatidylation activity of the phospholipase D reached an optimum and then declined abruptly. Alcohol concentrations required for maximal transphosphatidylation reaction generally decreased with increasing hydrophobicities of the alcohols. Nevertheless 1‐butanol and 4‐chloro‐l‐butanol were the most efficient co‐substrates, sharing identical optimal conditions. Transphosphatidylation works at the cost of phosphatidic acid formation. Phosphatidic acid itself was transformed to diacylglycerol, probably by a contaminating phosphatidic acid phosphohydrolase.