Details

Autor(en) / Beteiligte

• Kim, Min-Kyung
• Sisson, Gary
• Stoltz, Don

Titel

Ichnovirus Infection of an Established Gypsy Moth Cell Line

Ist Teil von

• Journal of general virology, 1996-09, Vol.77 (9), p.2321-2328

Ort / Verlag

England: Soc General Microbiol

Erscheinungsjahr

1996

Quelle

MEDLINE

Beschreibungen/Notizen

• Department of Microbiology & Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7 Author for correspondence: Don Stoltz. Fax + 1 902 494 5125, e-mail mail dstoltz{at}is.dal.ca In the present study, a lepidopteran cell line (Ld-652Y, from the gypsy moth, Lymantria dispar ) exposed to Hyposoter fugitivus polydnavirus (HfPV) was found to display a variety of cytopathic effects. These included a transient inhibition of cell proliferation, rounding up, aggregation and apoptosis. In addition, unusual paracrystalline structures appeared within the lumen of the rough endoplasmic reticulum; similar structures were observed in the spherulocytes of parasitized Malacosoma disstria . Following Coomassie Blue staining, two new cell-associated polypeptides were detected; one of these, an 8 kDa polypeptide, could also be observed following exposure of Ld-652Y cells to media taken from infected cultures or to cell-free haemolymph from parasitized M. disstria . After a period of 2–4 weeks, the L. dispar cell cultures were observed to largely recover from the effects of exposure to virus, and resumed proliferation; ‘transformed’ cell populations tended to form aggregates, and adhered less tightly to the substrate. Viral DNA was stably maintained in all recovered cell lines, possibly in chromosomally integrated form. Received 23 November 1995; accepted 26 April 1996.