FEBS letters, 1996-09, Vol.393 (1), p.101-104
1996
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Details
- Autor(en) / Beteiligte
- Titel
- Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinases (TIMP)-2
- Ist Teil von
- FEBS letters, 1996-09, Vol.393 (1), p.101-104
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 1996
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Membrane type 1-matrix metalloproteinase (MT1-MMP) initiates the activation of the zymogen progelatinase A/72-kDa type IV collagenase by cleavage of the Asn 66-Leu peptide bond. We previously pointed out that MT1-MMP possesses a unique amino acid sequence Arg-Arg-Lys-Arg 111 which is a potential recognition sequence for furin-like proteases ( Nature, 370 (1994) 61–65). Here, using a recombinant MT1-MMP expressed in Escherichia coli we demonstrated that furin specifically cleaves MT1-MMP between Arg 111-Tyr in vitro, which resulted in a stimulation of progelatinase A-activation function. Tissue inhibitor of metalloproteinases (TIMP)-2 inhibited activation of progelatinase A by forming a stable complex with activated MT1-MMP.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/0014-5793(96)00861-7Titel-ID: cdi_proquest_miscellaneous_78310987
- Format
- –
- Schlagworte
- Activation, Arginine, Binding Sites, Enzyme Activation, Enzyme Precursors - antagonists & inhibitors, Furin, Gelatinases - antagonists & inhibitors, Glycoproteins - pharmacology, Matrix metalloproteinase, Matrix Metalloproteinases, Membrane-Associated, Metalloendopeptidases - antagonists & inhibitors, Metalloendopeptidases - metabolism, MT1-MMP, MT1-MMP, membrane type 1-matrix metalloproteinase, Phenylalanine - analogs & derivatives, Phenylalanine - pharmacology, Protease Inhibitors - metabolism, Protease Inhibitors - pharmacology, Proteins - metabolism, Proteins - pharmacology, Recombinant Fusion Proteins - metabolism, Subtilisins - pharmacology, Thiophenes - pharmacology, TIMP, tissue inhibitor of metalloproteinases, Tissue Inhibitor of Metalloproteinase-2, Tissue Inhibitor of Metalloproteinases, Tyrosine