Details

Autor(en) / Beteiligte

• de Zeeuw, C.I.
• Holstege, J.C.
• Calkoen, F.
• Ruigrok, T.J.H.
• Voogd, J.

Titel

A new combination of WGA-HRP anterograde tracing and GABA immunocytochemistry applied to afferents of the cat inferior olive at the ultrastructural level

Ist Teil von

• Brain research, 1988-05, Vol.447 (2), p.369-375

Ort / Verlag

London: Elsevier B.V

Erscheinungsjahr

1988

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• In order to identify cerebellar terminals in the cat inferior olive which contain γ-aminobutyric acid (GABA), a technique was developed combining anterograde transport of wheatgerm agglutinine-conjugated horseradish (WGA-HRP) with gold-immunocytochemistry. With this technique both the HRP reaction product and the immunogold labelling can be visualized in a single ultrathin section. Our results suggest that most, if not all the WGA-HRP-labelled cerebellar terminals in the rostal medial accessory olive (MAO) and the rostral principal olive (PO) are GABAergic. In an additional experiment the GABAergic innervation of the rostral MAO was studied in combination with WGA-HRP anterograde tracing from the rostral mesencephalon. In this case the WGA-HRP-labelled terminals were never found to be GABA-positive.