Cell, 1988-07, Vol.54 (2), p.275-283
1988
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Details
- Autor(en) / Beteiligte
- Titel
- SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene
- Ist Teil von
- Cell, 1988-07, Vol.54 (2), p.275-283
- Ort / Verlag
- Cambridge, MA: Elsevier Inc
- Erscheinungsjahr
- 1988
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110–114, the product of the retinoblastoma susceptibility gene ( Rb). T and p110–114 coprecipitated in each case, implying that the proteins are complexed with each other. Substitution and internal deletion mutants of T that contain structural alterations in a ten residue, transformation-controlling domain failed to complex with p110–114. In contrast, T mutants bearing structural changes outside of this domain bound to p110–114. These results are consistent with a model for transformation by SV40 which, at least in part, involves T/p110–114 complex formation and the perturbation of Rb protein and/or T function.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/0092-8674(88)90559-4Titel-ID: cdi_proquest_miscellaneous_78306617
- Format
- –
- Schlagworte
- Animals, Antibodies, Monoclonal - immunology, Antigens, Polyomavirus Transforming - genetics, Antigens, Polyomavirus Transforming - immunology, Biological and medical sciences, Cell Line, Cell physiology, Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes, Disease Susceptibility, Electrophoresis, Polyacrylamide Gel, Eye Neoplasms - genetics, Fundamental and applied biological sciences. Psychology, Haplorhini, Immunoassay, Molecular and cellular biology, Mutation, Neoplasm Proteins - genetics, Neoplasm Proteins - immunology, Neoplasm Proteins - metabolism, Phosphoproteins - genetics, Phosphoproteins - immunology, Phosphoproteins - metabolism, Retinoblastoma - genetics, Simian virus 40 - genetics, Transfection