Details

Autor(en) / Beteiligte

• KÖLLER, MANFRED
• KÖNIG, WOLFGANG
• BROM, JOACHIM
• RAULF, MONIKA
• GROSS-WEEGE, WILHELM
• ERBS, GUNTHER
• MÜLLER, FRITZ E.

Titel

Generation of Leukotrienes from Human Polymorphonuclear Granulocytes of Severely Burned Patients

Ist Teil von

• The journal of trauma, 1988-06, Vol.28 (6), p.733-740

Ort / Verlag

Baltimore, MD: Williams & Wilkins

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• The Ca ionophore A23187-induced leukotriene (LT) release (LTC4, LTB4, 20-OH-LTB4, 20-COOH-LTB4) of human PMNʼs from severely burned patients (n=6) was studied by reversed-phase HPLC. The patientsʼ granulocytes demonstrated a decrease (to zero levels) in LT generation postburn. The level of generated LTʼs resembled that of healthy donors when the patients recovered from their trauma (after day 40 postburn). In contrast, the granulocytes of patients who finally succumbed to their injuries showed poor responsiveness over the total time. An enhanced LTC4 production by granulocytes correlated with an increase in eosinophils within the granulocyte fraction. In addition, the reduced LTB4 production was accompanied by an enhanced LTB4 metabolism to biologically less active products (ω-oxidated metabolites). Thus, the capacity of patientsʼ PMNʼs to release chemotactic substances was further decreased. The onset of this PMN dysfunction correlated with the onset of invasive microbial growth as determined by the quantitative bacterial analysis of full-thickness biopsy specimens. Our data provide evidence that the altered mediator release of patientsʼ PMNʼs is closely related to a depressed host defense.