Details

Autor(en) / Beteiligte

• Morera, A.M.
• Cochet, C.
• Keramidas, M.
• Chauvin, M.A.
• de Peretti, E.
• Benahmed, M.

Titel

Direct regulating effects of transforming growth factor β on the leydig cell steroidogenesis in primary culture

Ist Teil von

• Journal of steroid biochemistry, 1988, Vol.30 (1), p.443-447

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

1988

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The effect of transforming growth factor β on testicular steroidogenesis was studied by using a model of immature porcine Leydig cells cultured in a chemically defined medium. Leydig cells were cultured in the presence of human or porcine purified TGFβ and the following parameters were measured: cell proliferation, LH/hCG binding, and hCG-stimulated steroid hormone productions (DHEA, DHEAS and testosterone). Whereas TGFβ from the two sources had no effect on Leydig cell multiplication, it markedly inhibited LH/hCG binding, hCG-stimulated DHEA and DHEAS in a time- and dose-dependent manner. The maximal inhibitory effect of this peptide on LH/hCG binding (65% decrease), hCG-stimulated DHEA (77% decrease) and DHEAS (92% decrease) productions was observed with 2 ng/ml for 48 h of treatment. In contrast, TGFβ exerted a biphasic effect on hCG-stimulated testosterone production: stimulating (110% increase) until 2 ng/ml and inhibiting (35% decrease) for higher concentrations. [ 125I) TGFβ was cross-linked to Leydig cells using disuccinimidyl suberate; cells affinity labelled with [ 125I]TGFβ exhibit a major labelled band of approx 280 kDa, which has the properties expected from a TGFβ receptor. These data demonstrate that TGFβ is a direct potent regulator of Leydig cell steroidogenic function and its effects are probably mediated via a specific receptor.