Details

Autor(en) / Beteiligte

• Moţa, Gabriela
• Moraru, Ion
• Nicolae, Mariana
• Moraru, Ioan

Titel

The Fc receptor for IgA expression and affinity on lymphocytes and macrophages

Ist Teil von

• Molecular immunology, 1988-02, Vol.25 (2), p.95-101

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

1988

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The interaction between the Fc receptor for IgA (FcR α) and human secretory IgA (sIgA) was studied in a heterologous system by using rabbit alveolar macrophages or splenic lymphocytes. The capacity of FcR α-bearing cells to interact with the ligand via the Fc region was demonstrated. The proportion of FcR α-bearing lymphocytes and macrophages was found to be 11 and 17%, respectively. The cell-bound radiolabelled ligand showed a release half-time of 70 min for lymphocytes and 45 min for macrophages. The equilibrium constant ( K) and the maximum number of ligand molecules bound per cell ( n) in mean values was 5.2 ± 0.05 × 10 8 M −1 and 3.3 ± 0.15 × 10 5 molecules per cell, respectively. Increased percentages of FcR α-bearing cells and of ligand density per cell were obtained after stimulation by lung inflammation. A significant increase of the K value was recorded with macrophages from rabbits with lung acute inflammation (mean value 7.3 ±0.21 + 10 8 M −1) while the increase with lymphocytes was not significant. The calculation method of K and n is discussed.