Details

Autor(en) / Beteiligte

• Lansdorp, Peter M.
• Verwoerd, Nico P.
• van de Rijke, Frans M.
• Dragowska, Visia
• Little, Marie-Térèse
• Dirks, Roeland W.
• Raap, Anton K.
• Tanke, Hans J.

Titel

Heterogeneity in Telomere Length of Human Chromosomes

Ist Teil von

• Human molecular genetics, 1996-05, Vol.5 (5), p.685-691

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

1996

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Vertebrate chromosomes terminate in variable numbers of T2AG3 nucleotide repeats. In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C3TA2)3 peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis.