Details

Autor(en) / Beteiligte

• Shibata, D
• Steczko, J
• Dixon, J E
• Andrews, P C
• Hermodson, M
• Axelrod, B

Titel

Primary structure of soybean lipoxygenase L-2

Ist Teil von

• The Journal of biological chemistry, 1988-05, Vol.263 (14), p.6816-6821

Ort / Verlag

Bethesda, MD: Elsevier Inc

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• The nucleotide sequence of soybean lipoxygenase-2 cDNA has been determined, and the complete amino acid sequence of the enzyme has been deduced. Limited direct amino acid sequence data for lipoxygenase-2 protein support this assignment and exclude mRNA representing lipoxygenase-1 and -3. Lipoxygenase 2 has a molecular weight of 97,036 and contains 865 amino acid residues, in contrast to the isozymes, lipoxygenase-1 and -3, which are known to contain 838 and 859 amino acid residues, respectively. Despite significant differences in behavior between these three isozymes, the amino acid sequences of lipoxygenase-1 and -3 are 81 and 74% identical to lipoxygenase-2, respectively. A region of 40 amino acid residues containing a cluster of six histidines and two tyrosines, which is highly conserved in all three isozymes, is discussed as a possible iron-binding region.