Details

Autor(en) / Beteiligte

• LIEN, E. A
• SOLHEIM, E
• KVINNSLAND, S
• UELAND, P. M

Titel

Identification of 4-hydroxy-N-desmethyltamoxifen as a metabolite of tamoxifen in human bile

Ist Teil von

• Cancer research (Chicago, Ill.), 1988-04, Vol.48 (8), p.2304-2308

Ort / Verlag

Philadelphia, PA: American Association for Cancer Research

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• The occurrence of tamoxifen metabolites in bile was investigated in a 57-year-old female patient receiving chronic treatment with tamoxifen. In bile treated with beta-glucuronidase, two major peaks were detected using a chromatographic system developed for the quantitation of tamoxifen metabolites in human serum. One sharp peak coeluted with 4-hydroxy-tamoxifen whereas a second broad peak eluted slightly ahead of tamoxifen and was separated from all major serum metabolites. This latter peak was identified as the cis (about 30%) and trans (about 70%) isomers of 4-hydroxy-N-desmethyltamoxifen. The identification was based on (a) coelution with authentic standard on reversed-phase chromatography and formation of fluorescent material after photoactivation, (b) a molecular ion (M + 1)+ of 374 m/z as determined with liquid chromatography-mass spectrometry, and (c) a fragmentogram identical to that of the authentic standard, as obtained by gas chromatography-mass spectrometry.