Details

Autor(en) / Beteiligte

• Blazar, Bruce R.
• Widmer, Michael B.
• Soderling, Christine C.B.
• Urdal, David L.
• Gillis, Steven
• Robison, Leslie L.
• Vallera, Daniel A.

Titel

Augmentation of Donor Bone Marrow Engraftment in Histoincompatible Murine Recipients by Granulocyte/Macrophage Colony-Stimulating Factor

Ist Teil von

• Blood, 1988-02, Vol.71 (2), p.320-328

Ort / Verlag

Washington, DC: Elsevier Inc

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• T cell depletion of donor bone marrow can prevent graft v host disease (GVHD) in human and murine marrow graft recipients. However, engraftment in the recipient may be compromised as a consequence of donor marrow T cell depletion. The effect of recombinant murine granulocyte/ macrophage colony-stimulating factor (rmu GM-CSF) on engraftment and hematologic reconstitution was evaluated in a murine allogeneic bone marrow transplantation (BMT) model involving T cell depletion of marrow. Before transplantation into irradiated mice differing at major and minor histocompatibility loci, rmu GM-CSF was preincubated with T cell-depleted donor marrow. When low degrees of engraftment were noted in control recipients, treatment of donor marrow with high concentrations of rmu GM-CSF led to enhanced engraftment. Ex vivo donor graft incubation with rmu GM-CSF or a single in vivo injection of rmu GM-CSF were both effective means of promoting engraftment. When the engraftment rate in control recipients was high, rmu GM-CSF did not have an identifiable effect. Only slight increases in hematologic recovery were detected regardless of the rate of engraftment. Neither post-BMT survival nor marrow stem cell capacity was affected by rmu GM-CSF incubation. Furthermore, growth factor administration did not have a significant effect on the incidence of GVHD in recipients of non-T cell-depleted bone marrow/ splenocyte preparations. In vitro natural killer-mediated target cell lysis was not altered by incubation of effector cells with rmu GM-CSF. These results demonstrate the potential of ex vivo rmu GM-CSF treatment of donor marrow to facilitate engraftment across extensive histocompatibility barriers.©1988 by Grune & Stratton, Inc. 0006-4971/88/7102-0002S3.00/0