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Expression of atrial natriuretic peptide by third-trimester placental cytotrophoblasts in women
Ist Teil von
Biology of reproduction, 1996-04, Vol.54 (4), p.834-840
Ort / Verlag
Madison, WI: Society for the Study of Reproduction
Erscheinungsjahr
1996
Quelle
MEDLINE
Beschreibungen/Notizen
Atrial natriuretic peptide (ANP) is a vasoactive molecule with a potential role in modulating uteroplacental blood flow. We
examined the expression of ANP in the third-trimester fetomaternal interface by immunohistochemistry performed on sections
of placental tissue as well as by reverse transcriptase-polymerase chain reaction (RT-PCR) and RIA performed on isolated,
purified term trophoblasts. Results from immunohistochemical studies on ten normal term placental samples revealed labeling
of extravillous interstitial and cytotrophoblastic shell trophoblasts. The trophoblastic identity of the cells was determined
by labeling of adjacent serial sections with anti-cytokeratin antibody. Labeling of decidual tissue was also observed in some
samples (3 of 10 samples), whereas villous trophoblastic tissue showed no labeling. RT-PCR analysis of RNA extracted from
isolated third-trimester trophoblasts revealed the presence of a 0.45-kb and band corresponding to ANP mRNA. Sequencing of
the amplified cDNA confirmed its identity. RIA on lysates of isolated trophoblasts revealed the presence of ANP at a level
of 41 pg +/- 8 pg/10(7) cells (n = 4; range = 16.8-50.4 pg/10(7) cells). These results indicate that placental cytotrophoblastic
tissue is a source of ANP. This molecule might play a role in the regulation of uteroplacental and/or fetoplacental blood
flow.