Details

Autor(en) / Beteiligte

• CLAUDIO, P. P
• DE LUCA, A
• HOWARD, C. M
• BALDI, A
• FIRPO, E. J
• KOFF, A
• PAGGI, M. G
• GIORDANO, A

Titel

Functional analysis of pRb2/p130 interaction with cyclins

Ist Teil von

• Cancer research (Chicago, Ill.), 1996-05, Vol.56 (9), p.2003-2008

Ort / Verlag

Philadelphia, PA: American Association for Cancer Research

Erscheinungsjahr

1996

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The retinoblastoma (Rb) family consists of the tumor suppressor pRb and related proteins p107 and pRb2/p130. Ectopic expression of pRb and p107 results in a growth arrest of sensitive cells in the G1 phase of the cell cycle. We demonstrated here that the growth-suppressive properties of pRb2/p130 were also specific for the G1 phase. The A-, E-, and D-type cyclins as well as transcription factor E2F1 and the E1A viral oncoprotein were able to rescue the pRb2/p130-mediated G1 growth arrest in SAOS-2 cells. The rescue with cyclins A and E correlated with their physical interaction with pRb2/p130, which surprisingly has been found to occur over all phases of the cell cycle. The phosphorylation status as well as the kinase activity associated with pRb2/p130 dramatically increased near the G1-S-phase transition. This suggests that, like the other Rb family members, pRb and p107, the phosphorylation of pRb2/p130 is controlled by the cell cycle machinery and that pRb2/p130 may indeed be another key G1-S-phase regulator.