Details

Autor(en) / Beteiligte

• Hofmann, Kathryn J
• Neeper, Michael P
• Markus, Henry Z
• Brown, Darron R
• Muller, Martin
• Jansen, Kathrin U

Titel

Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae

Ist Teil von

• Journal of general virology, 1996-03, Vol.77 (3), p.465-468

Ort / Verlag

England: Soc General Microbiol

Erscheinungsjahr

1996

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• 1 Department of Virus and Cell Biology, Merck Research Laboratories, Sumneytown Pike, West Point, Pennsylvania 19486 2 Division of Infectious Diseases, Indiana University School of Medicine and Roudebush Veterans Administration Medical Center, Indianapolis, Indiana 46202-5124 and 3 Loyola University Medical Center, Maywood, Illinois 60153, USA The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences for L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into viruslike particles was demonstrated. * Author for correspondence. Fax +1 215 652 2142. e-mail Kathrin-Jansen@Merck.com Received 22 August 1995; accepted 31 October 1995.