FEBS letters, 1987-12, Vol.225 (1), p.139-144
1987
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Generation of OH radical during enzymatic reduction of 9,10-anthraquinone-2-sulphonate Can semiquinone decompose hydrogen peroxide?
- Ist Teil von
- FEBS letters, 1987-12, Vol.225 (1), p.139-144
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 1987
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Spin-trapping experiments have shown that 9, 10-anthraquinone-2-sulphonate (AQS) can be reduced enzymatically with NADPH-cytochrome P-450 reductase (FP) producing OH radicals. It has been revealed for the first time that the radical anion of AQS cannot decompose H 2O 2 as indicated by flash-photolysis data. From the experimental results obtained it follows that an enzyme system containing NADPH, AQS and FP produces both accumulation of H 2O 2 from molecular oxygen and reduction of Fe 3+ to Fe 2+ which decomposes H 2O 2 catalytically via a Fenton reaction.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/0014-5793(87)81146-8Titel-ID: cdi_proquest_miscellaneous_77886840
- Format
- –
- Schlagworte
- Anthraquinones - metabolism, Antineoplastic agents, AQS, 9,10-anthraquinone-2-sulphonate (Na salt), Benzoquinones, Biological and medical sciences, Cyclic N-Oxides, DMPO, 5,5-dimethylpyrroline N-oxide, DMSO, dimethyl sulphoxide, Electron Spin Resonance Spectroscopy, FP, NADPH-cytochrome P-450 reductase, General aspects, Hydrogen peroxide, Hydrogen Peroxide - metabolism, Hydroxides - metabolism, Hydroxyl Radical, Kinetics, Medical sciences, NADP - metabolism, NADPH-cytochrome P-450 reductase, NADPH-Ferrihemoprotein Reductase - metabolism, Oxidation-Reduction, Oxygen - metabolism, Pharmacology. Drug treatments, Photolysis, Quinone, Quinones - metabolism, Reaction mechanism, SOD, superoxide dismutase, Spin Labels