Details

Autor(en) / Beteiligte

• Kemp, Bruce E.
• Moseley, Jane M.
• Rodda, Christine P.
• Ebeling, Peter R.
• Richard E. H. Wettenhall
• Stapleton, David
• Diefenbach-Jagger, Hannelore
• Ure, Fiona
• Michelangeli, Valdo P.
• Simmons, Hollis A.
• Raisz, Lawrence G.
• Martin, T. John

Titel

Parathyroid Hormone-Related Protein of Malignancy: Active Synthetic Fragments

Ist Teil von

• Science (American Association for the Advancement of Science), 1987-12, Vol.238 (4833), p.1568-1570

Ort / Verlag

Washington, DC: The American Association for the Advancement of Science

Erscheinungsjahr

1987

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Peptides corresponding to the amino-terminal region of the parathyroid hormone-related protein (PTHrP) of humoral hypercalcemia of malignancy were synthesized. A 34-amino acid peptide, PTHrP(1-34), was two to four times more potent than bovine or human PTH(1-34) in bioassays promoting the formation of adenosine 3$^{\prime}$,5$^{\prime}$-monophosphate (cAMP) and plasminogen activator activity in osteogenic sarcoma cells and adenylate cyclase activity in chick kidney membranes. Like parathyroid hormone itself, in which the activity resides in the first 34 residues, PTHrP peptides of less than 30 residues from the amino terminus showed substantially reduced activity. PTHrP(1-34) had only 6% of the potency of bovine PTH(1-34) in promoting bone resorption in vitro. PTHrP(1-34) strongly promoted the excretion of cAMP and phosphorus and reduced the excretion of calcium in the isolated, perfused rat kidney consistent with the symptoms seen in malignant hypercalcemia.