Details

Autor(en) / Beteiligte

• Cha, Xian Yuan
• Xu, Heng
• Ni, Qing
• Partilla, John S.
• Rice, Kenner C.
• Matecka, Dorota
• Calderon, Silvia N.
• Porreca, Frank
• Lai, Josephine
• Rothman, Richard B.

Titel

Opioid peptide receptor studies. 4. Antisense oligodeoxynucleotide to the delta opioid receptor delineates opioid receptor subtypes

Ist Teil von

• Regulatory peptides, 1995-10, Vol.59 (2), p.247-253

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1995

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Prior work in our laboratory has identified putative subtypes of δ ( δ cx-1, δ cx-2, δ ncx-1, δ ncx-2) and κ 2 ( κ 2a and κ 2b) receptors. Previous studies showed that chronic (three day) i.c.v. administration of antisense oligodeoxynucleotide to the cloned δ opioid receptor selectively decreased [ 3H][ d-Ala 2, d-Leu 5]enkephalin binding to the δ ncx site, not the δ cx-2 site. The present study extends this work by demonstrating that δ antisense DNA selectively affects the δ ncx-2 site sparing the other putative δ receptor subtypes and κ 2 receptor subtypes. This selectivity is not due to anatomically specific effects of δ antisense DNA since autoradiograms show that δ binding is reduced in all regions of the brain after chronic i.c.v. administration of δ antisense DNA. These data strongly suggest that the δ cx-1, δ cx-2, δ ncx-1, κ 2a and κ 2b binding sites are different proteins than the δ ncx-2 binding site, which, based on its sensitivity to δ antisense DNA, is synonymous to the cloned δ opioid receptor. Viewed collectively, these data suggest that administration of δ antisense DNA, and by extension other receptor-selective antisense DNA, is a powerful approach to distinguishing between postulated receptor subtypes.