Details

Autor(en) / Beteiligte

• Hayes, P.S
• Blom, K
• Feng, P
• Lewis, J
• Strockbine, N.A
• Swaminathan, B

Titel

Isolation and characterization of a beta-D-glucuronidase-producing strain of Escherichia coli serotype O157:H7 in the United States

Ist Teil von

• Journal of Clinical Microbiology, 1995-12, Vol.33 (12), p.3347-3348

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• A phenotypic variant of Escherichia coli serotype O157:H7 (G5101) was isolated from a patient with bloody diarrhea. Strain G5101 does not ferment sorbitol but is beta-D-glucuronidase and urease positive. Serotyping and colony hybridization using a serotype-specific DNA probe confirmed that the isolate was O157:H7. G5101 produces Shiga-like toxins I and II and contains an eae gene that is highly conserved in the O157:H7 serotype. This strain would have been missed by laboratories that screen for the sorbitol-negative, beta-D-glucuronidase-negative phenotype in isolating E. coli O157:H7 from clinical and food specimens.