Details

Autor(en) / Beteiligte

• Ema, Masatsugu
• Matsushita, Natsuki
• Sogawa, Kazuhiro
• Ariyama, Takeshi
• Inazawa, Johji
• Nemoto, Takayuki
• Ota, Minoru
• Oshimura, Mitsuo
• Fujii-Kuriyama, Yoshiaki

Titel

Human Arylhydrocarbon Receptor: Functional Expression and Chromosomal Assignment to 7p21

Ist Teil von

• Journal of biochemistry (Tokyo), 1994, Vol.116 (4), p.845-851

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911–917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human×mouse or Chinese hamster hybrid cell DNAs.