Somatic cell and molecular genetics, 1995-07, Vol.21 (4), p.255-263
1995
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Details
- Autor(en) / Beteiligte
- Titel
- Molecular cloning of the human gene SUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation
- Ist Teil von
- Somatic cell and molecular genetics, 1995-07, Vol.21 (4), p.255-263
- Ort / Verlag
- United States
- Erscheinungsjahr
- 1995
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designated SUVCC3 to denote solar UV cross-complementing gene number 3.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0740-7750DOI: 10.1007/BF02255780Titel-ID: cdi_proquest_miscellaneous_77722690
- Format
- –
- Schlagworte
- Adult, Animals, Bromodeoxyuridine, Cell Line, Cell Survival - radiation effects, Cloning, Molecular, Cross-Linking Reagents, DNA - metabolism, DNA - radiation effects, DNA Repair, Female, Fibroblasts - cytology, Fibroblasts - metabolism, Fibroblasts - radiation effects, Humans, Kinetics, Light, Photolysis, Proteins - metabolism, Proteins - radiation effects, Ranidae, Skin - cytology, Skin - metabolism, Skin - radiation effects, Sunlight, Time Factors, Transfection, Ultraviolet Rays