Details

Autor(en) / Beteiligte

• Kobayashi, K.
• Watanabe, J.
• Umeda, F.
• Masakado, M.
• Ono, Y.
• Taniguchi, S.
• Yanase, T.
• Hashimoto, T.
• Sako, Y.
• Nawata, H.

Titel

Metabolism of Oxidized Glycated Low-Density Lipoprotein in Cultured Bovine Aortic Endothelial Cells

Ist Teil von

• Hormone and metabolic research, 1995-08, Vol.27 (8), p.356-362

Ort / Verlag

Germany

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract The serum concentration of glycated low-density lipoprotein (gLDL) is increased in individuals with diabetes mellitus, which may be a contributing factor to the increased incidence of atherosclerosis in this population. Given the importance of oxidized LDL (oLDL) in atherosclerosis and that vascular endothelial cells express receptors for oLDL, oxidized glycated LDL (ogLDL) was prepared in vitro and its binding and degradation by cultured bovine aortic endothelial cells were examined. Glycation of native LDL (nLDL) isolated from normal human subjects was performed by incubation with 20 mM glucose at 37°C for 3 days, and ogLDL was prepared by oxidation of gLDL with 1 µM CuSO 4 at 37°C for 12 hours. The electrophoretic mobility and thiobarbituric acid reactive substance (TBARS) value of ogLDL were greater than those of nLDL and gLDL. Both binding and degradation of ogLDL by cultured endothelial cells also were significantly greater than for nLDL and gLDL. Degradation of nLDL by endothelial cells was completely inhibited by ogLDL, whereas degradation of acetylated LDL was not inhibited by nLDL or ogLDL. Thus, the binding and degradation of ogLDL by endothelial cells do not appear to be mediated by the scavenger receptor. Although the exact mechanism is not clear, it appears that vascular endothelial cells may play a protective role against atherosclerosis by removing potential atherogenic lipoproteins.