Details

Autor(en) / Beteiligte

• Cancela, J.M.
• Bertrand, N.
• Beley, A.

Titel

Involvement of cAMP in the Regulation of High Affinity Choline Uptake by Rat Brain Synaptosomes

Ist Teil von

• Biochemical and biophysical research communications, 1995-08, Vol.213 (3), p.944-949

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1995

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The role of cAMP in the regulation of the high affinity choline uptake (HACU) was investigated in resting and KCl - stimulated rat brain synaptosomes. The data indicate that the permeable cAMP analogue, monobutyryl-8-bromo cAMP, increased dose-dependently the HACU in resting synaptosomes. Treatments of resting synaptosomes by oxotremorine, quinacrine, and promethazine resulted in a reduced cAMP formation with a concomitant decrease of HACU. The reduction of HACU could be completely counteracted by the monobutyryl-8-bromo cAMP following oxotremorine treatment and was only partially inhibited in quinacrine and promethazine treated resting synaptosomes. KCl stimulation resulted in a significant increase in cAMP formation and HACU by the synaptosomes. The different profile of data obtained following the previous pharmacological treatments in KCl - stimulated synaptosomes suggests that both cAMP and phospholipase A 2 pathways may act synergistically to coordinate the neuronal choline incorporation.