Details

Autor(en) / Beteiligte

• Laidler, V. (University of Warwick, Coventry, UK.)
• Chaddock, A.M
• Knott, T.G
• Walker, D
• Robinson, C

Titel

A SecY homolog in Arabidopsis thaliana: sequence of a full-length cDNA clone and import of the precursor protein into chloroplasts

Ist Teil von

• The Journal of biological chemistry, 1995-07, Vol.270 (30), p.17664-17667

Ort / Verlag

United States

Erscheinungsjahr

1995

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Proteins are translocated across the thylakoid membrane by two distinct pathways in higher plant chloroplasts, one of which is related to prokaryotic Sec-dependent translocation mechanisms. SecY is an essential, hydrophobic component of the membrane-bound translocase complex in bacteria, and we report here the nucleotide sequence of a full-length cDNA encoding a homolog of SecY from Arabidopsis thaliana. The predicted protein of 651 residues includes an amino-terminal extension of approximately 120 residues when compared with other SecY proteins. The deduced sequence of the mature protein, cpSecY, is 41% identical with SecY from Synechococcus and 33% identical with the Escherichia coli protein. The extension serves to target the protein into chloroplasts; transcription-translation of the cDNA yields a 58-kDa precursor protein which is imported into pea chloroplasts, processed to a product of 46 kDa, and targeted into the thylakoid membrane