Neuroscience letters, 1995-04, Vol.189 (3), p.139-142
1995
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- Autor(en) / Beteiligte
- Titel
- Muscarinic cholinergic receptors in dorsal root ganglia of chick embryo: a radioligand binding and immunocytochemical study
- Ist Teil von
- Neuroscience letters, 1995-04, Vol.189 (3), p.139-142
- Ort / Verlag
- Shannon: Elsevier Ireland Ltd
- Erscheinungsjahr
- 1995
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- The presence and micro-anatomical localization of muscarinic cholinergic receptors were assessed in dorsal root ganglia of chick embryo during development using radioligand binding and immunocytochemical techniques, respectively. The non-selective muscarinic cholinergic receptor radioligand [ 3H]quinuclidinyl benzilate was specifically bound to sections of chick dorsal root ganglia with a dissociation constant value ( K d) of 0.75 ± 0.02 nM and a maximum density of binding sites ( B max) of 7.2 ± 0.5 fmol/mg tissue. [ 3H]Quinuclidinyl benzilate binding was partially sensitive to pirenzepine displacement. This suggests that muscarinic cholinergic receptors expressed by dorsal root ganglia of chick embryo at least in part belong to the M 1 muscarinic receptor subtype. Immunocytochemical analysis confirmed the presence of muscarinic receptors in the ganglia. These findings suggest that neurons of dorsal root ganglia, which are known to express cholinergic markers such as choline acetyltransferase, acetylcholinesterase and high affinity choline uptake, are also cholinoceptive.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0304-3940eISSN: 1872-7972DOI: 10.1016/0304-3940(95)11474-BTitel-ID: cdi_proquest_miscellaneous_77410933
- Format
- –
- Schlagworte
- Animals, Binding, Competitive - drug effects, Biological and medical sciences, Brain Chemistry - drug effects, Chick development, Chick Embryo, Development. Senescence. Regeneration. Transplantation, Dorsal root ganglia, Fundamental and applied biological sciences. Psychology, Ganglia, Spinal - metabolism, Immunohistochemistry, Kinetics, Muscarinic cholinergic receptors, Quinuclidinyl Benzilate, Radioligand Assay, Receptors, Muscarinic - drug effects, Receptors, Muscarinic - metabolism, Sensory neurons, Vertebrates: nervous system and sense organs