Details

Autor(en) / Beteiligte

• Zhang, Ji-Nan
• Wilks, James E.
• Billadello, Joseph J.

Titel

Characterization of a Nuclear Protein That Interacts with Regulatory Elements in the Human B Creatine Kinase Gene

Ist Teil von

• The Journal of biological chemistry, 1995-07, Vol.270 (27), p.16134-16139

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• The B creatine kinase gene is regulated by an array of positive and negative cis-elements in the 5′-flanking DNA that function in both muscle and nonmuscle cells. In C2C12 myogenic cells M and B creatine kinase mRNAs are coordinately up-regulated in the early stages of myogenesis and then undergo distinct regulatory programs. The B creatine kinase gene is down-regulated in the late stages of myogenesis as M creatine kinase becomes the predominant species in mature myotubes. Sequences between −92 and +80 of the B creatine kinase gene confer a regulated pattern of expression to chimeric plasmids that closely resembles the time course of expression of the endogenous B creatine kinase gene in C2C12 cells undergoing differentiation. We show that sequences within the first exon of the B creatine kinase gene are important for the developmental regulation of the gene in C2C12 cells and that these sequences bind a nuclear protein that shows a similar tissue-specific distribution and developmentally regulated expression to that of the endogenous B creatine kinase gene.