Details

Autor(en) / Beteiligte

• Goodman, Matthew M.
• McCullough, Jerry L.
• Biren, Catherine A.
• Barr, Ronald J.

Titel

A Model of Human Melanoma in Cyclosporine-Immunosuppressed Rats

Ist Teil von

• Journal of investigative dermatology, 1987-02, Vol.88 (2), p.141-144

Ort / Verlag

Danvers, MA: Elsevier Inc

Erscheinungsjahr

1987

Quelle

MEDLINE

Beschreibungen/Notizen

• A human malignant melanoma maintained in athymic nude mice has been successfully implanted and grown in cyclosporine (Cys-)immunosuppressed Lewis rats. Suspended melanoma cells (106) or solid tumor sections measuring 2–4mm in diameter were implanted s.c. in rats receiving parenteral Cys doses of 15–50 mg/kg each day for 1 weeks, and 3 times per week thereafter. Eighty-five percent of solid tumor section implanted in animals receiving 25 mg/kg resulted in tumor growth, whereas no tumors grew from cell suspension injection sites. The average maximum tumor growth rate was 2cm3/day, with a doubling time of 8 days. Tumors retained pretransplant gross and microscopic morphology, karyotype, and labeling index. Possible advantages of this model over the athymic nude mouse include greater longevity, larger animal and tumor size, and less stringent aseptic environmental requirements. This model may prove useful for further study of the pathophysiology of melanoma and for testing of new antimelanoma therapies.