Details

Autor(en) / Beteiligte

• Blasco, M J
• López Bernal, A
• Turnbull, A C

Titel

11 beta-Hydroxysteroid dehydrogenase activity of the human placenta during pregnancy

Ist Teil von

• Hormone and metabolic research, 1986-09, Vol.18 (9), p.638-641

Ort / Verlag

Germany

Erscheinungsjahr

1986

Quelle

MEDLINE

Beschreibungen/Notizen

• A method for the quantitative estimation of 11 beta-hydroxysteroid dehydrogenase activity (11 beta-HSD; EC.1.1.146) in human placental homogenates is described. This method is based on the separation of cortisol and cortisone by high performance liquid chromatography after extraction from homogenates incubated in the presence of cortisol and NADP. 11 beta-HSD activity (pmol/g wet weight per min) averaged 900 +/- 150 (mean +/- SEM) at 10 +/- 2 weeks of gestation, 915 +/- 35 at 17 +/- 2 weeks and 790 +/- 42 at 40 +/- 2 weeks, thus supporting the view that the placenta is an effective barrier to materno-fetal cortisol transfer throughout gestation.