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Odontoblast processes in dentin revealed by fluorescent Di-I
Ist Teil von
The journal of histochemistry and cytochemistry, 1995-02, Vol.43 (2), p.159-168
Ort / Verlag
United States: SAGE Publications
Erscheinungsjahr
1995
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
There has been controversy about the length and structure of the odontoblast process
within dentin since the earliest histologic studies of teeth. Our objective was to
use the fluorescent carbocyanine dye Di-I combined with a new gelatin embedment
procedure and confocal microscopy to determine the structure and extent of
odontoblast processes in developing and mature rat teeth, injured rat molars,
reparative dentin, and adult monkey teeth. We found that odontoblast processes do not
extend into outer dentin or to the dentin-enamel junction except during early stages
of development. Those in innervated regions of crown are long and straight, whereas
those in roots are extensively branched and shorter. Cavity injury to crown dentin
caused odontoblast fragments to be aspirated into outer dentin. In reparative dentin
the odontoblast processes were branched and similar to those in roots. We used
photoconversion and electron microscopy to show that Di-I fills the entire
odontoblast after gelatin embedment, including the cytoplasm. This is a different
type of carbocyanine staining from any previously reported, and it also stains other
cells in adjacent hard tissues such as bone and cementum. The Di-I-gelatin method is
a new way to use carbocyanine dyes. It has enabled us to solve a long-standing
controversy about the histology of teeth, and it should be useful for many other
studies of cell structure.