Details

Autor(en) / Beteiligte

• Sheppard, Karen E.
• Punder, John W.

Titel

Cortisol 17β acid, transcortin, and the heterogeneity of rat brain glucocorticoid receptors

Ist Teil von

• Journal of steroid biochemistry, 1986-08, Vol.25 (2), p.285-288

Ort / Verlag

Oxford: Elsevier B.V

Erscheinungsjahr

1986

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Binding of tracer or competing steroids to transcortin can compromise specificity studies on receptors for adrenal steroids. Recently Alexis et al.[l] have used Cortisol 17β acid at high concentrations to prevent steroid binding to any transcortin possibly contaminating rat brain cytosol preparations. On the basis of limited specificity studies of [ 3H]dexamethasone and [ 3H]corticosterone binding under such conditions, it was claimed that binding sites for the two steroids are indistinguishable, and it is thus unnecessary to invoke distinct binding sites for each glucocorticoid [1]. We have extended these competition studies in the presence of Cortisol 17β acid, and shown that in rat hippocampus Type I, corticosterone-preferring glucocorticoid receptors can be clearly distinguished both from transcortin and from Type II, dexamethasone-binding glucocorticoid receptors.