Gene, 2001-02, Vol.264 (2), p.187-196
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene
- Ist Teil von
- Gene, 2001-02, Vol.264 (2), p.187-196
- Ort / Verlag
- Netherlands
- Erscheinungsjahr
- 2001
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chromatin assembly during DNA replication (Smith and Stillman, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involved in interaction with histone H3 and H4, is critical to the CAF-1 nucleosome assembly activity. In this study, we sequenced a 96-kb genomic DNA region that includes a 42.8-kb CAF-1 p150 subunit gene (CHAF1A), and a 41.1-kb EEN gene. A scripted bioinformatics analysis pipeline (research agent) has been set up to annotate the BAC sequence with a set of integrated algorithms. The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promoter region is characterized by deletional analyses, revealing a potential repressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR which resulted more splicing forms than computational prediction.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1119DOI: 10.1016/S0378-1119(01)00335-3Titel-ID: cdi_proquest_miscellaneous_76964532
- Format
- –
- Schlagworte
- 3T3 Cells, Alternative Splicing, Amino Acid Sequence, Animals, Base Sequence, CAF-1 protein, CHAF1A gene, Chromatin Assembly Factor-1, Chromosomal Proteins, Non-Histone, Computational Biology, COS Cells, DNA - chemistry, DNA - genetics, DNA, Complementary - genetics, DNA-Binding Proteins - genetics, Genes - genetics, Humans, Luciferases - genetics, Luciferases - metabolism, Mice, Molecular Sequence Data, Promoter Regions, Genetic - genetics, Protein Subunits, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factors