Details

Autor(en) / Beteiligte

• van Maarsseveen, A
• Alberts, C
• van der Schoot, J
• van Royen, E
• Hens, C
• Mullink, H
• de Groot, J

Titel

Radionuclides in detecting active granuloma formation. Gallium-67 scintigraphy and histopathology with autoradiographic findings

Ist Teil von

• Annals of the New York Academy of Sciences, 1986, Vol.465 (1), p.427-434

Ort / Verlag

United States

Erscheinungsjahr

1986

Quelle

Access via Wiley Online Library

Beschreibungen/Notizen

• Granuloma formation studies were performed on lungs of guinea pigs sensitized with FCA over 2 to 17 months. Prolonged time of sensitization revealed more granulomatous pulmonary tissue. An intravenous booster of FCA in the animals that had been sensitized for 3 months yielded enhanced granuloma formation within 5 days. The histopathology of these lungs was comparable with that seen in lungs of animals after 17 months of sensitization without booster. Enhanced local proliferation of macrophages, measured by [3H]thymidine incorporation and autoradiography, was seen in the lungs of the animals that had received boosters. Moreover, 67Ga scintigraphy was strongly positive in these animals. Scintigraphy of cell suspensions of pulmonary tissue from these animals showed that 67Ga was predominantly taken up (quantitatively as well as qualitatively) by the alveolar macrophages. Cell suspensions of sarcoidosis patients, prepared in the same way, showed only a low level of 67Ga uptake, one comparable to that of the pulmonary cell suspensions of the sensitized animals that had not received boosters. It is suggested that a negative scintigraphy in patients with chronic pulmonary granulomatous disorders could be (partly) explained by the absence of activated macrophages.