Details

Autor(en) / Beteiligte

• Lin, Keh-Liang
• Hsieh, Kue-Hsiung
• Thomas, Wayne R.
• Chiang, Bor-Luen
• Chua, Kaw-Yan

Titel

Characterization of Der p V allergen, cDNA analysis, and IgE-mediated reactivity to the recombinant protein

Ist Teil von

• Journal of allergy and clinical immunology, 1994-12, Vol.94 (6), p.989-996

Ort / Verlag

New York, NY: Mosby, Inc

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• A λ gt11 library for cDNA from Dermatophagoides pteronyssinus was screened by plaque immunoassay, and a number of related clones that produced IgE-binding proteins were isolated. Their sequences were homologous to that of a cDNA described previously, which contained a reading frame encoding a 17 kd polypeptide and which as determined by serologic analysis corresponded to a protein with relative molecular mass of 14 kd in mite extracts. The cDNA from the λ gt11 clones was truncated so it was used to obtain longer clones from a λ gt10 library. Analysis of the sequence of these clones showed that the allergen now designated Der p V is produced from a 132-residue polypeptide, which has a putative 19-residue leader peptide and a 113-residue mature protein. This would have a molecular weight of 14 kd, corresponding to that found in mite extracts. IgE binding studies with the λ gt11 clone and a fusion of the mature sequence in a pGEX construct showed that it reacted with 50% of allergic sera. Further studies with skin tests indicated that it caused reactions in about 60% of patients with asthma and 29% of those with allergic rhinitis alone. (J ALLERGY CLIN IMMUNOL 1994;94:989-96.)