Details

Autor(en) / Beteiligte

• Rensen, Patrick C.N.
• Love, William G.
• Taylor, Peter W.

Titel

In vitro interaction of zinc(II)-phthalocyanine-containing liposomes and plasma lipoproteins

Ist Teil von

• Journal of photochemistry and photobiology. B, Biology, 1994-10, Vol.26 (1), p.29-35

Ort / Verlag

Switzerland: Elsevier B.V

Erscheinungsjahr

1994

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• We have studied the interaction of small unilamellar liposomes containing zinc(II)-phthalocyanine (Zn-Pc) with human plasma lipoproteins. High-, low- and very low-density lipoproteins (HDL, LDL and VLDL) were purified from plasma and combined in amounts reflecting their natural abundance in plasma. After short periods of incubation at 37 °C, the bulk of Zn-Pc was incorporated into HDL and LDL; very little 14C-labelled palmitoyl oleoyl phosphocholine, the most abundant phospholipid in the formulation, was associated with lipoproteins. When liposomes were incubated in pooled plasma, 73%–85% of Zn-Pc and 27%–34% of radiolabelled phospholipid were recovered with HDL and LDL, indicating a possible role for plasma lipid transfer proteins in the incorporation of phospholipid into lipoproteins. Some Zn-Pc was also found in association with VLDL. The buoyant density of Zn-Pc liposomes increased in a dose-dependent fashion when the particles were incubated with plasma, and it is suggested that this was due, at least in part, to opsonization of liposomes by plasma proteins.