Details

Autor(en) / Beteiligte

• Bain, Gretchen
• Maandag, Els C.Robanus
• Izon, David J.
• Amsen, Derk
• Kruisbeek, Ada M.
• Weintraub, Bennett C.
• Krop, Ian
• Schlissel, Mark S.
• Feeney, Ann J.
• van Roon, Marian
• van der Valk, Martin
• te Riele, Hein P.J.
• Berns, Anton
• Murre, Cornelis

Titel

E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements

Ist Teil von

• Cell, 1994-12, Vol.79 (5), p.885-892

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1994

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (−/−) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I μ RAG-1, mb-1, CD19, and λ5 transcripts are dramatically reduced in fetal livers of E2A (−/−) mice, B29 and μ° transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (−/−) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation.