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Antigen‐induced changes in B cell subsets in lymph nodes: analysis by dual fluorescence flow cytofluorometry
Ist Teil von
European journal of immunology, 1986, Vol.16 (7), p.829-834
Ort / Verlag
Weinheim: WILEY‐VCH Verlag GmbH
Erscheinungsjahr
1986
Link zum Volltext
Quelle
Wiley Online Library All Journals
Beschreibungen/Notizen
Changes in the representation and surface phenotype of defined B cell subsets in murine lymph nodes stimulated with keyhole limpet hemocyanin or sheep red blood cells have been analyzed by two‐color immunofluorescence fluorocytometric analysis. Shortly after immunization with either antigen there is a dramatic increase in both the frequency and absolute number of IgM+, IgD+ B cells, which is followed by the formation of germinal centers. Germinal center cells, as soon as they appear on day 3 after primary immunization, bind high levels of peanut agglutinin, bear low levels of surface IgM but no detectable surface IgD, and are characterized by lack of staining with MEL‐14, a monoclonal antibody which recognizes a lymphocyte surface receptor involved in lymphocyte homing. The level of I‐A and H‐2K region‐encoded surface antigens on early germinal center cells is higher than on PNAlo B cells. During the first 7 days of the germinal centers there is a progressive decrease in the average level of H‐2K but not of Ia antigens. A similar decrease was observed for ThB. It is confirmed that the germinal center cell population contains the majority of antigen‐binding cells in the stimulated lymph node. These findings indicate that B cells are recruited nonspecifically to antigen‐stimulated lymph nodes, and that the antigen‐specific cells then selectively participate in the formation of germinal centers where they undergo specific differentiation events.