Details

Autor(en) / Beteiligte

• OIKARINEN, A.I.
• REUNALA, T.
• ZONE, J.J.
• KIISTALA, U.
• UITTO, J.

Titel

Proteolytic enzymes in blister fluids from patients with dermatitis herpetiformis

Ist Teil von

• British journal of dermatology (1951), 1986-03, Vol.114 (3), p.295-302

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1986

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• SUMMARY Proteolytic enzymes may be involved in blister formation in dermatitis herpetiformis (DH). We have examined collagenase, gelatinase and elastase‐like enzyme activities in fluids collected from spontaneous blisters and from suction blisters raised on developing DH‐lesions induced by application of potassium iodide. Control suction blisters were raised on unaffected DH‐skin and on healthy volunteers. High enzyme activities were found in spontaneous blisters, and suction blister fluids obtained from developing lesions showed increased levels of gelatinase and elastase‐like enzymes. Inhibitor studies revealed that a part of the elastase‐like enzyme activity might be derived from inflammatory cells. Gel filtration chromatography disclosed two separate elastase‐like enzymes which, as they had high molecular weights, could be either enzyme‐inhibitor complexes or aggregates.