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PCR‐RFLP studies on chromosome 3p in formaldehyde‐fixed, paraffin‐embedded cervical cancer tissues
Ist Teil von
International journal of cancer, 1994-09, Vol.58 (6), p.787-792
Ort / Verlag
New York: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
1994
Link zum Volltext
Quelle
Wiley Blackwell Single Titles
Beschreibungen/Notizen
Loss of heterozygosity (LOH) has been extensively studied on the short arm of chromosome 3, and functional proofs have been obtained defining a tumor‐suppressor locus at 3p21‐22. We examined 31 paraffin‐embedded cervical cancer samples for LOH, using 5 PCR‐primer pairs, located around 3p21. Allele loss was found in 19 out of the 27 informative samples (70%) while 13 out of 23 informative samples (56%) had LOH located at 3p2l‐22. More of the human papillomavirus (HPV)‐positive samples had LOH compared to the HPV‐negative samples, giving only a weak association between loss of allele and HPV integration. Modifications of the DNA in the formaldehyde‐fixed samples were detected, and further studies will be required to clarify how such artifacts may affect restriction fragment length polymorphism (RFLP) studies on fixed tissues.