Gene, 1994-09, Vol.146 (2), p.285-289
1994
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- Autor(en) / Beteiligte
- Titel
- A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter
- Ist Teil von
- Gene, 1994-09, Vol.146 (2), p.285-289
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1994
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- We have produced a truncated form of the human estrogen receptor (hER) as a fusion protein with glutathione S-transferase (GST) in Spodoptera frugiperda (Sf) cells using the baculovirus expression vector (BEV) system. The protein is correctly produced and can be purified from crude whole-cell extracts by a single-step, batch-wise affinity-purification procedure. We show that this GST-hER fusion protein binds at its DNA-binding site specifically and in a hormone-inducible manner. Furthermore, we used the purified hER to analyze the complex estrogen response element ( ERE) in the promoter of the oxytocin-encoding gene.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1119eISSN: 1879-0038DOI: 10.1016/0378-1119(94)90307-7Titel-ID: cdi_proquest_miscellaneous_76686123
- Format
- –
- Schlagworte
- affinity chromatography, Baculoviridae - genetics, baculovirus, Base Sequence, COUP-TF-like factor, DNA - metabolism, Genetic Vectors - chemistry, Genetic Vectors - genetics, Glutathione S-transferase fusion protein, Glutathione Transferase - chemistry, Glutathione Transferase - genetics, Glutathione Transferase - metabolism, Humans, Molecular Sequence Data, Receptors, Estrogen - chemistry, Receptors, Estrogen - isolation & purification, Receptors, Estrogen - metabolism, Recombinant Fusion Proteins - chemistry, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, Spodoptera frugiperda