Details

Autor(en) / Beteiligte

• Minichiello, Liliana
• Remondelli, Paolo
• Cigliano, Stefania
• Bonatti, Stefano
• Leone, Arturo

Titel

Interactions of nuclear proteins from uninduced, induced and superinduced HeLa cells with metal regulatory elements MRE3 and 4 of the human metallothionein IIa-encoding gene

Ist Teil von

• Gene, 1994-06, Vol.143 (2), p.289-294

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1994

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Transcriptional activation of metallothionein (MT)-encoding genes ( MT) is regulated during heavy metal induction by short non-identical repeats, termed ‘metal regulatory elements’ ( MRE), present in multiple imperfect copies in MT promoter regions of eukaryotes. Using mobility shift assays, we have studied the interaction between the human MRE 3 and 4 regions (h MRE3/4) of the MTIIa promoter and nuclear proteins from uninduced and Cd 2+-induced HeLa cells, and from Cd 2+-superinduced H454 cells, a HeLa-derived Cd 2+-resistant cell isolate which overexpresses h MTIIa after exposure to metal. A specific complex with a similar electrophoretic mobility was formed in all three extracts. Dialysis of the extracts using EDTA inhibited the formation of the complexes, which could be reconstituted only after the addition of Zn 2+. UV cross-linking analyses of the specific complexes formed by the three nuclear extracts interacting with the h MRE3/4 region revealed that in all of them polypeptides were present having similar electrophoretic mobilities and different molecular masses. Mobility shift assays showed no major differences in the binding of nuclear proteins from induced or uninduced cells. Proposed models of activation of metal-induced MT transcription are discussed.