Details

Autor(en) / Beteiligte

• Lok, Si
• Kaushansky, Kenneth
• Holly, Richard D
• Kuijper, Joseph L
• Lofton-Day, Catherine E
• Oort, Pieter J
• Grant, Francis J
• Heipel, Mark D
• Burkhead, Steve K
• Kramer, Janet M
• Bell, L. Anne
• Sprecher, Cindy A
• Blumberg, Hal
• Johnson, Rebecca
• Prunkard, Donna
• Ching, Andrew F. T
• Mathewes, Shannon L
• Bailey, Mason C
• Forstrom, John W
• Buddle, Michele M
• Osborn, Sherri G
• Evans, Simon J
• Sheppard, Paul O
• Presnell, Scott R
• O'Hara, Patrick J
• Hagen, Fredrick S
• Roth, Gerald J
• Foster, Donald C

Titel

Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo

Ist Teil von

• Nature (London), 1994-06, Vol.369 (6481), p.565-568

Ort / Verlag

London: Nature Publishing

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• The major regulator of circulating platelet levels is believed to be a cytokine termed thrombopoietin. It is thought to be a lineage-specific cytokine affecting the proliferation and maturation of committed cells resulting in the production of megakaryocytes and platelets. Despite considerable efforts by a number of laboratories, the unequivocal identification of thrombopoietin has proven elusive. Here we report the functional cloning of a murine complementary DNA encoding a ligand for the receptor encoded by the c-mpl proto-oncogene (c-Mpl). The encoded polypeptide has a predicted molecular mass of 35,000 (M(r) 35K). The protein has a novel two-domain structure with an amino-terminal domain homologous with erythropoietin and a carboxy-terminal domain rich in serine, threonine and proline residues and containing seven potential N-linked glycosylation sites. Intraperitoneal injections of mice with recombinant protein increase circulating platelet levels by greater than fourfold after 7 days. These results along with those presented in the accompanying report strongly suggest that the ligand for c-Mpl is thrombopoietin.