Details

Autor(en) / Beteiligte

• Smith, G.A. (Queensland Univ., St Lucia (Australia). Gehrmann Lab.)
• Young, P.L
• Rodwell, B.J
• Kelly, M.A
• Storie, G.J
• Farrah, C.A. (Queensland Dept. of Primary Industries, Yeerongpilly (australia). Animal Research Inst.)
• Mattick, J.S. (Queensland Univ., St Lucia (Australia). Centre for Molecular Biology and Biotechnology)

Titel

Development and trial of a bovine herpesvirus 1 - thymidine kinase deletion virus as a vaccine [cattle]

Ist Teil von

• Australian veterinary journal, 1994-03, Vol.71 (3), p.65-70

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1994

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5'-iodo-deoxy-uridine the virus was analysed by hybridisation with 3 differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.