Details

Autor(en) / Beteiligte

• Bertram, J.
• Killian, M.
• Brysch, W.
• Schlingensiepen, K.H.
• Kneba, M.

Titel

Reduction of erbB2 Gene-Product in Mamma Carcinoma Cell Lines by erbB2 mRNA-Specific and Tyrosine Kinase Consensus Phosphorothioate Antisense Oligonucleotides

Ist Teil von

• Biochemical and biophysical research communications, 1994-04, Vol.200 (1), p.661-667

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1994

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• A considerable reduction of up to 75% at the protein level of the erbB2 gene product was observed using phosphorothioate antisense oligonucleotides directed against specific sequences of the erbB2 mRNA. Antisense oligonucleotides used were the 14-mer (translation start region) and the 17-mer (3′ translated region) all-phosphorothioate oligonucleotides (S-ODNs). Sense or random sequences were used as a control. The greatest reduction at the erbB2 protein level was obtained after a 24h incubation with a single dose of 2 μM antisense S-ODN. The erbB2-mRNA-specific and the tyrosine kinase consensus antisense S-ODNs were comparably effective in inhibiting erbB2 expression. Addition of the transfection reagent DOTAP diminished the efficiency of erbB2 protein reduction by antisense S-ODNs after an incubation period of 24h but was more effective after 48h compared to the application of antisense S-ODNs alone.