Details

Autor(en) / Beteiligte

• van Kooten, Cees
• Gaillard, Claude
• Galizzi, Jean‐Pierre
• Hermann, Patrice
• Fossiez, François
• Banchereau, Jacques
• Blanchard, Dominique

Titel

B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40

Ist Teil von

• European journal of immunology, 1994-04, Vol.24 (4), p.787-792

Ort / Verlag

Weinheim: WILEY‐VCH Verlag GmbH

Erscheinungsjahr

1994

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• The expression of CD40 ligand (CD40L) on activated T cells (CD4+ T cell clone MT9) is diminished when the T cells are cultured in the presence of B cells. This effect, observed both with normal tonsil B cells and with the B cell line JY, was detected after 6 h and sustained at least until 18 h of co‐culture. Analysis of mRNA showed that CD40L mRNA levels were not modified after 6 h, but were significantly down‐regulated after 18 h of co‐culture with B cells. Although CD40L expression could not be detected by a CD40‐Fc chimera, the molecule was still expressed at the membrane as shown with a polyclonal antiserum against CD40L (anti‐TRAP). In addition, T cells activated in the presence of B cells were stained by a polyclonal antiserum against CD40, without the appearance of CD40 mRNA. These results indicated that a soluble form of CD40 (sCD40) bound to the expressed CD40L on T cells. The existence of sCD40 was confirmed by detection of sCD40 in B cell supernatants using a specific enzyme‐linked immunosorbent assay. Collectively, these data show that B cells can regulate the expression of CD40L on activated T cells at least by two different mechanisms.