Hormone and metabolic research, 1994-01, Vol.26 (1), p.43-45
1994
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Enzyme-Linked Immunosorbent Assay (ELISA) Method for Screening of Non-Classical Steroid 21-Hydroxylase Deficiency
- Ist Teil von
- Hormone and metabolic research, 1994-01, Vol.26 (1), p.43-45
- Ort / Verlag
- Stuttgart: Thieme
- Erscheinungsjahr
- 1994
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary Morning salivary 17-hydroxyprogesterone (17-OHP) determination is a useful method to diagnose nonclassical 21-hydroxylase deficiency (NC 21-OHD). In ethnic groups with high frequency of NC 21-OHD, mass screening is thought to be necessary. A pilot screening test for NC 21-OHD was performed by measuring morning salivary 17-OHP by enzyme-linked immunosorbent assay (ELISA). Two individuals were found NC 21-OHD from 230 subjects. Results of this ELISA were well correlated with those of established radioimmunoassay. ELISA can be performed in any laboratory because of non-radioactivity and the procedure is very easy by the use of a microplate. This ELISA is suitable for large scale population studies. We conclude that morning salivary 17-OHP determination by ELISA is a useful method for NC 21-OHD screening test.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0018-5043eISSN: 1439-4286DOI: 10.1055/s-2007-1000770Titel-ID: cdi_proquest_miscellaneous_76422897
- Format
- –
- Schlagworte
- 17-alpha-Hydroxyprogesterone, Adrenal Hyperplasia, Congenital, Adult, Biological and medical sciences, Endocrinology, Enzyme-Linked Immunosorbent Assay - methods, Female, Humans, Hydroxyprogesterones - analysis, Investigative techniques, diagnostic techniques (general aspects), Male, Mass Screening, Medical sciences, Originals Clinical, Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques, Radioimmunoassay, Saliva - chemistry