Archives of biochemistry and biophysics, 1985-09, Vol.241 (2), p.432-437
1985
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Details
- Autor(en) / Beteiligte
- Titel
- Insulin stimulates generation of intracellular mediators in rat heart
- Ist Teil von
- Archives of biochemistry and biophysics, 1985-09, Vol.241 (2), p.432-437
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1985
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Insulin treatment of rats results in an increased amount or activity of insulin mediators in heart muscle. The mediators stimulated mitochondrial pyruvate dehydrogenase and inhibited glucagon-stimulated adenylate cyclase. The mediators were copurified by ultrafiltration, ethanol extraction, Dowex cation-exchange, and QAE-Sephadex anion-exchange chromatography. The activities of the two mediators were separated by Sephadex G-10 chromatography. Fasting rats for 72 h diminished the mediator response to insulin treatment. These results, taken together with previous reports, indicate that insulin generates a number of mediators which have a ubiquitous tissue distribution. The activity of these mediators, like insulin responsiveness, is altered by the metabolic state of the animal.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-9861eISSN: 1096-0384DOI: 10.1016/0003-9861(85)90567-3Titel-ID: cdi_proquest_miscellaneous_76363640
- Format
- –
- Schlagworte
- Adenylyl Cyclase Inhibitors, Animals, Applied sciences, Chromatography, Affinity, Enzyme Activation, Exact sciences and technology, Glucagon - pharmacology, Insulin - pharmacology, Male, Myocardium - metabolism, Other techniques and industries, Pyruvate Dehydrogenase Complex - analysis, Rats, Rats, Inbred Strains