Journal of leukocyte biology, 1993-12, Vol.54 (6), p.564-571
1993
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- Autor(en) / Beteiligte
- Titel
- Both mannose and beta-glucan receptors are involved in phagocytosis of unopsonized, heat-killed Saccharomyces cerevisiae by murine macrophages
- Ist Teil von
- Journal of leukocyte biology, 1993-12, Vol.54 (6), p.564-571
- Ort / Verlag
- Bethesda, MD: Society for Leukocyte Biology
- Erscheinungsjahr
- 1993
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We studied the involvement of lectin‐like receptors in phagocytosis of unopsonized heat‐killed yeast (Saccharomyces cerevisiae) by murine macrophagelike cell lines and murine peritoneal resident macrophages. For this purpose we used a technique that allowed us to discriminate ingested and adsorbed heat‐killed yeast. The internalization can be partly inhibited by soluble polyosides such as laminarin (β‐glucan) or a‐ mannan. However, when they were used together (0.4 mg/ml α‐mannan and 0.4 mg/ml laminarin), almost complete inhibition of phagocytosis was obtained. These observations suggest that phagocytosis of unopsonized heat‐ killed yeast by murine macrophage‐like cell lines as well as murine peritoneal resident macrophages is mediated by both mannose and β‐glucan receptors. The respective activity of these two types of receptors is a function of in vitro cell differentiation. To achieve maximal phagocytosis of unopsonized heat‐killed yeast, coexpression of both mannose and β‐glucan receptors is required.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0741-5400eISSN: 1938-3673DOI: 10.1002/jlb.54.6.564Titel-ID: cdi_proquest_miscellaneous_76097488
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Cell Death, Cell Line, Fundamental and applied biological sciences. Psychology, Fundamental immunology, Glucan Endo-1,3-beta-D-Glucosidase - pharmacology, Glucans - metabolism, Hot Temperature, Immunobiology, laminarin, Lectins, C-Type, lectin‐like receptor, Macrophages - immunology, Macrophages, Peritoneal - immunology, Mannans - pharmacology, mannose receptor, Mannose-Binding Lectins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Monocytes, macrophages, Myeloid cells: ontogeny, maturation, markers, receptors, Phagocytosis - drug effects, Polysaccharides - pharmacology, Receptors, Cell Surface - physiology, Receptors, Immunologic - physiology, receptor‐ mediated phagocytosis, Saccharomyces cerevisiae, Saccharomyces cerevisiae - cytology, Saccharomyces cerevisiae - immunology, α‐mannan, β ‐glucan receptor