Details

Autor(en) / Beteiligte

• Carvalhal, R.F.
• Machado, D.S.
• Mendes, R.K.
• Almeida, A.L.J.
• Moreira, N.H.
• Piazetta, M.H.O.
• Gobbi, A.L.
• Kubota, L.T.

Titel

Development of a disposable amperometric biosensor for salicylate based on a plastic electrochemical microcell

Ist Teil von

• Biosensors & bioelectronics, 2010-06, Vol.25 (10), p.2200-2204

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• The use of an amperometric biosensor for rapid salicylate determination in blood is described. Photolitography was used to make gold electrodes on a polyester film. The plastic microcell was characterized using cyclic voltammetry to demonstrate the electrochemical performance of the system. The biosensor was constructed by immobilizing salicylate hydroxylase onto the working electrode of the plastic electrochemical microcell. The optimized working conditions were 0.1 mol L −1 phosphate buffer at pH 7.6 with 0.5 mmol L −1 of NADH and 300 mV vs. Au as the applied potential. The resulting biosensor exhibited a high sensitivity (97.4 nA/mmol L −1 salicylate) and an adequate linear response range (1.2 × 10 −4 to 1.0 × 10 −3 mol −1). The biosensor performance was verified by determining salicylate in spiked blood samples and the results were statistically equivalent to the values obtained from the standard Trinder spectrophotometric method, with a 95% confidence level. This study shows the potential development of a portable, inexpensive and disposable device for point-of-care monitoring.