Journal of neurochemistry, 1985-04, Vol.44 (4), p.1111-1115
1985
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Details
- Autor(en) / Beteiligte
- Titel
- Assay of Brain Calmodulin Levels Using High‐Performance Liquid Chromatography
- Ist Teil von
- Journal of neurochemistry, 1985-04, Vol.44 (4), p.1111-1115
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1985
- Quelle
- Wiley Online Library All Journals
- Beschreibungen/Notizen
- : A simple, sensitive, and efficient HPLC method for the determination of calmodulin levels in brain tissue extracts is described. The assay is linear with respect to both calmodulin and protein concentrations. The specificity and validity of this assay for calmodulin is demonstrated by parallel radioimmunoassay determinations which give equivalent results. Determination of calmodulin levels in various brain regions revealed a high concentration of this protein in the hypothalamus, by comparison to other areas examined.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3042eISSN: 1471-4159DOI: 10.1111/j.1471-4159.1985.tb08732.xTitel-ID: cdi_proquest_miscellaneous_75986762
- Format
- –
- Schlagworte
- Animals, Biochemistry and metabolism, Biological and medical sciences, Brain Chemistry, Calmodulin, Calmodulin - analysis, Central nervous system, Chromatography, High Pressure Liquid, Fundamental and applied biological sciences. Psychology, HPLC, Hypothalamus - analysis, Male, Radioimmunoassay, Rats, Rats, Inbred Strains, Tissue Distribution, Vertebrates: nervous system and sense organs